Dp power chip7/7/2023 ![]() ![]() Vlckova M, Kalman F, Schwarz MA (2008) Pharmaceutical applications of isoelectric focusing on microchip with imaged UV detection. ![]() ![]() Guillo C, Karlinsey JM, Landers JP (2007) On-chip pumping for pressure mobilization of the focused zones following microchip isoelectric focusing. Mao Q, Pawliszyn J (1999) Demonstration of isoelectric focusing on an etched quartz chip with UV absorption imaging detection. Hofmann O, Che D, Cruickshank KA, Muller UR (1999) Adaptation of capillary isoelectric focusing to microchannels on a glass chip. Silvertand LHH, Torano JS, van Bennekom WP, de Jong GJ (2008) Recent developments in capillary isoelectric focusing. Shen Y, Berger SJ, Anderson GA, Smith RD (2000) High-efficiency capillary isoelectric focusing of peptides. Tang Q, Harrata AK, Lee CS (1996) High-resolution capillary isoelectric focusing-electrospray ionization mass spectrometry for hemoglobin variants analysis. Three kinds of microchips that have been successfully developed using standard soft lithography technology are described in detail. The chips are designed for use in the iCE280 analyzer (Convergent Bioscience Inc., Toronto), for capillary-based IEF where UV-WCID is employed for analyzing the separation performance. We have developed polydimethylsiloxane (PDMS) microchips for IEF applications where UV-WCID is employed for evaluating the separation performance. Fluorescence- and UV absorbance-based WCID have been successfully adapted in glass and PDMS microchips. WCID is more favorable than single-point detection because the latter requires the focused bands to be mobilized and thus adds more complexity to the design and operation of such microchips. Both single-point and whole column imaging detection (WCID) methods have been employed for analyzing the separation performance in a microchip. ![]() With the advancement in micromachining technology, microchip format IEF has attracted significant attention. Isoelectric focusing (IEF) is considered as an attractive separation technique for biologically amphoteric compounds (e.g., proteins and peptides) based on their isoelectric point (pI). ![]()
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